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streptavidin coated poly  (Thermo Fisher)


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    Thermo Fisher streptavidin coated poly
    Streptavidin Coated Poly, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/streptavidin+poly/Streptavidin/bio_rxiv__64898__2026__04__18__719423-224-4-26
    Average 99 stars, based on 1 article reviews
    streptavidin coated poly - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: BRD-810 is a highly selective MCL1 inhibitor with optimized in vivo clearance and robust efficacy in solid and hematological tumor models
    Article Snippet: Biotinylated anti-BAK (Santa Cruz, sc-1035) or anti-Bim (Cell Signaling, 2933) antibody was added and incubated for 2 h. Wells were washed once with 250 μl of 1× PBST. .. Streptavidin poly(horseradish peroxidase) (Thermo Fisher) was diluted to 20 ng ml −1 in Odyssey blocking buffer plus 0.05% Triton X-100 and 100 μl was added to each well of the ELISA plate. ..

    Article Title: BRD-810 is a highly selective MCL1 inhibitor with optimized in vivo clearance and robust efficacy in solid and hematological tumor models.
    Article Snippet: Biotinylated anti-BAK (Santa Cruz, sc-1035) or anti-Bim (Cell Signaling, 2933) antibody was added and incubated for 2 h. Wells were washed once with 250 μl of 1× PBST. .. Streptavidin poly(horseradish peroxidase) (Thermo Fisher) was diluted to 20 ng ml−1 in Odyssey blocking buffer plus 0.05% Triton X-100 and 100 μl was added to each well of the ELISA plate. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: BRD-810 is a highly selective MCL1 inhibitor with optimized in vivo clearance and robust efficacy in solid and hematological tumor models
    Article Snippet: Biotinylated anti-BAK (Santa Cruz, sc-1035) or anti-Bim (Cell Signaling, 2933) antibody was added and incubated for 2 h. Wells were washed once with 250 μl of 1× PBST. .. Streptavidin poly(horseradish peroxidase) (Thermo Fisher) was diluted to 20 ng ml −1 in Odyssey blocking buffer plus 0.05% Triton X-100 and 100 μl was added to each well of the ELISA plate. ..

    Article Title: BRD-810 is a highly selective MCL1 inhibitor with optimized in vivo clearance and robust efficacy in solid and hematological tumor models.
    Article Snippet: Biotinylated anti-BAK (Santa Cruz, sc-1035) or anti-Bim (Cell Signaling, 2933) antibody was added and incubated for 2 h. Wells were washed once with 250 μl of 1× PBST. .. Streptavidin poly(horseradish peroxidase) (Thermo Fisher) was diluted to 20 ng ml−1 in Odyssey blocking buffer plus 0.05% Triton X-100 and 100 μl was added to each well of the ELISA plate. ..

    Amplification:

    Article Title: Sub-zeptomole Detection of Biomarker Proteins Using a Microfluidic Immunoarray with Nanostructured Sensors
    Article Snippet: .. The key to greatly increased sensitivity and ultralow LODs is premixing samples with biotinylated secondary antibodies (Ab 2 ) and streptavidin poly(horseradish peroxidase) (polyHRP, Pierce, from Thermo-Fisher, contains 400 HRPs per molecule) in large excess to form protein-Ab 2 -polyHRP bioconjugates to provide 400-fold HRPs amplification compared to a single HRP. ..

    Article Title: Sub-zeptomole Detection of Biomarker Proteins Using a Microfluidic Immunoarray with Nanostructured Sensors
    Article Snippet: .. The key to greatly increased sensitivity and ultra-low LODs is pre-mixing samples with biotinylated secondary antibodies (Ab2) and streptavidin poly(horseradish peroxidase) (polyPRP, Pierce, from Thermo-Fisher, contains 400 HRPs per molecule) in large excess to form protein-Ab2-polyHRP bioconjugates to provide 400-fold HRPs amplification compared to a single HRP. ..

    Saline:

    Article Title: Middle-Down and Chemical Proteomic Approaches to Reveal Histone H4 Modification Dynamics in Cell Cycle: Label-Free Semi-Quantification of Histone Tail Peptide Modifications Including Phosphorylation and Highly Sensitive Capture of Histone PTM Binding Proteins Using Photo-Reactive Crosslinkers
    Article Snippet: The samples were separated on NuPAGE 10% Bis-Tris gel (Invitrogen) and transferred onto poly(vinylidene difluoride) (PVDF) membranes by using iBlot apparatus (Invitrogen). .. The membranes were blocked with Tris-buffered saline (TBS) containing 0.1% Tween 20 and 1% bovine serum albumin (BSA), then hybridized with streptavidin–poly(horseradish peroxidase) conjugate (SA-HRP; Pierce Protein Biology Products, Rockford, IL, USA) in Can Get Signal Immunoreaction Enhancer Solution 1 (Toyobo Corp., Osaka, Japan) for two hours. .. The proteins were then detected by using Luminata Forte Western HRP substrate (EMD Millipore, Bellerica, MA, USA) with a LAS-3000 imager (FUJIFILM, Tokyo, Japan).



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    Principle of the PI3Kα activity assay and titration of its basic components GST-GRP1 and b-PIP 3 . A , table listing the key steps (1–5) of the assay. B , schematic illustration of the assay’s principle. The number of each individual step of the assay in ( B ) corresponds to the same number of the step shown in ( A ) (numbers are shown in red fonts , both in ( A ) and ( B ). In step 1, GST-GRP1 is immobilized to the walls of the plate. In step 2, in the absence of PI3Kα (negative control), the plates are incubated with b-PIP 3 alone. In the presence of PI3Kα, the enzymatic reactions are set up in separate tubes (step 2a), b-PIP 3 is added to the tubes post termination of the PI3Kα reaction (step 2b) and the mixture is added to the wells (step 2c), where PIP 3 (generated by PI3Kα) competes with b-PIP 3 (added exogenously) for binding to GST-GRP1. Next, <t>streptavidin-HRP</t> is added (step 3) to assess the amount of bound b-PIP 3 , followed by the addition of OPD (step 4), the specific substrate of HRP, used to generate a chromophore measured at 492 nm (step 5). C , the optimal amount of GST-GRP1 in the assay was determined by testing increasing concentrations of this component, followed by addition of 0.9 ng b-PIP 3 . Two replicates from n = 5. D , the optimal amount of b-PIP 3 was assessed by adding varying quantities of b-PIP 3 to glutathione-coated wells, preincubated with 4 μg GST-GRP1. Two replicates from n = 5. The individual data points displayed above represent the means of replicates in each experiment. The statistical significance of difference between groups was examined by one-way ANOVA, followed by Tukey’s multiple comparisons test. ns , no significance, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. Data were shown as mean ± SD. GRP, general receptor for phosphoinositide; GST, glutathione-S-transferase; PIP 3 , phosphatidylinositol-3,4,5-triphosphate; b-PIP 3 , biotin-phosphatidylinositol-3,4,5-triphosphate; HRP, horseradish peroxidase.
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    Principle of the PI3Kα activity assay and titration of its basic components GST-GRP1 and b-PIP 3 . A , table listing the key steps (1–5) of the assay. B , schematic illustration of the assay’s principle. The number of each individual step of the assay in ( B ) corresponds to the same number of the step shown in ( A ) (numbers are shown in red fonts , both in ( A ) and ( B ). In step 1, GST-GRP1 is immobilized to the walls of the plate. In step 2, in the absence of PI3Kα (negative control), the plates are incubated with b-PIP 3 alone. In the presence of PI3Kα, the enzymatic reactions are set up in separate tubes (step 2a), b-PIP 3 is added to the tubes post termination of the PI3Kα reaction (step 2b) and the mixture is added to the wells (step 2c), where PIP 3 (generated by PI3Kα) competes with b-PIP 3 (added exogenously) for binding to GST-GRP1. Next, streptavidin-HRP is added (step 3) to assess the amount of bound b-PIP 3 , followed by the addition of OPD (step 4), the specific substrate of HRP, used to generate a chromophore measured at 492 nm (step 5). C , the optimal amount of GST-GRP1 in the assay was determined by testing increasing concentrations of this component, followed by addition of 0.9 ng b-PIP 3 . Two replicates from n = 5. D , the optimal amount of b-PIP 3 was assessed by adding varying quantities of b-PIP 3 to glutathione-coated wells, preincubated with 4 μg GST-GRP1. Two replicates from n = 5. The individual data points displayed above represent the means of replicates in each experiment. The statistical significance of difference between groups was examined by one-way ANOVA, followed by Tukey’s multiple comparisons test. ns , no significance, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. Data were shown as mean ± SD. GRP, general receptor for phosphoinositide; GST, glutathione-S-transferase; PIP 3 , phosphatidylinositol-3,4,5-triphosphate; b-PIP 3 , biotin-phosphatidylinositol-3,4,5-triphosphate; HRP, horseradish peroxidase.

    Journal: The Journal of Biological Chemistry

    Article Title: A new functional assay reveals that membrane binding is critical for overactivation of the phosphoinositide 3-kinase H1047R mutant

    doi: 10.1016/j.jbc.2026.111207

    Figure Lengend Snippet: Principle of the PI3Kα activity assay and titration of its basic components GST-GRP1 and b-PIP 3 . A , table listing the key steps (1–5) of the assay. B , schematic illustration of the assay’s principle. The number of each individual step of the assay in ( B ) corresponds to the same number of the step shown in ( A ) (numbers are shown in red fonts , both in ( A ) and ( B ). In step 1, GST-GRP1 is immobilized to the walls of the plate. In step 2, in the absence of PI3Kα (negative control), the plates are incubated with b-PIP 3 alone. In the presence of PI3Kα, the enzymatic reactions are set up in separate tubes (step 2a), b-PIP 3 is added to the tubes post termination of the PI3Kα reaction (step 2b) and the mixture is added to the wells (step 2c), where PIP 3 (generated by PI3Kα) competes with b-PIP 3 (added exogenously) for binding to GST-GRP1. Next, streptavidin-HRP is added (step 3) to assess the amount of bound b-PIP 3 , followed by the addition of OPD (step 4), the specific substrate of HRP, used to generate a chromophore measured at 492 nm (step 5). C , the optimal amount of GST-GRP1 in the assay was determined by testing increasing concentrations of this component, followed by addition of 0.9 ng b-PIP 3 . Two replicates from n = 5. D , the optimal amount of b-PIP 3 was assessed by adding varying quantities of b-PIP 3 to glutathione-coated wells, preincubated with 4 μg GST-GRP1. Two replicates from n = 5. The individual data points displayed above represent the means of replicates in each experiment. The statistical significance of difference between groups was examined by one-way ANOVA, followed by Tukey’s multiple comparisons test. ns , no significance, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. Data were shown as mean ± SD. GRP, general receptor for phosphoinositide; GST, glutathione-S-transferase; PIP 3 , phosphatidylinositol-3,4,5-triphosphate; b-PIP 3 , biotin-phosphatidylinositol-3,4,5-triphosphate; HRP, horseradish peroxidase.

    Article Snippet: Glutathione-coated plates as well as streptavidin poly-HRP were from PIERCE/Thermo Fisher Scientific.

    Techniques: Activity Assay, Titration, Negative Control, Incubation, Generated, Binding Assay